An alternative nested-PCR assay for the detection of Toxoplasma gondii strains based on GRA7 gene sequences
نویسندگان
چکیده
منابع مشابه
Multiplex PCR for typing strains of Toxoplasma gondii.
A multiplex PCR assay was designed for multilocus strain typing of Toxoplasma gondii based on length polymorphism of five microsatellite markers. Eight T. gondii strains already sequenced at these five markers were used as control isolates. This method is simple, rapid, reproducible, and adapted to a large set of isolates.
متن کاملDetection of Toxoplasma gondii in Diabetic Patients Using the Nested PCR Assay via RE and B1 Genes.
BACKGROUND Toxoplasma gondii is an obligate intracellular protozoan parasite that exists worldwide. Various techniques have been developed for T. gondii detection. OBJECTIVES The aim of this study was the detection of T. gondii in diabetic patients with RE and B1 genes and the comparison of these two genes for diagnosis using the nested-PCR assay method. PATIENTS AND METHODS DNA samples fro...
متن کاملSelection of polymorphic peptides from GRA6 and GRA7 sequences of Toxoplasma gondii strains to be used in serotyping.
The evaluation of Toxoplasma gondii isolates obtained from geographical environments other than Europe and North America revealed the existence of atypical strains that are not included in the three archetypal clonal lineages (lineages I, II, and III). GRA6 and GRA7 are polymorphic genes that have been used for the genotyping of Toxoplasma. The coding regions of GRA6 and GRA7 from 49 nonarchety...
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Background: Shigella is among the most important human pathogenic microorganisms, infecting both humans and nonhuman and causing clinically severe diarrhea. Shigella must be enriched before detection, which is time-consuming.Objectives: To develop a sensitive, rapid, and specifc method for Shigella detection.Materials and Methods: S...
متن کاملdetection of toxoplasma gondii in diabetic patients using the nested pcr assay via re and b1 genes
conclusions our study demonstrated that the b1 gene, more so than the re gene, showed positive samples and can be used to detect toxoplasmosis, although the b1 gene, in comparison to the re gene, did not show any superiority of molecular diagnosing capability. results also showed that toxoplasma molecular detection methods can be used instead of routine serological detection methods in a clinic...
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ژورنال
عنوان ژورنال: Acta Tropica
سال: 2016
ISSN: 0001-706X
DOI: 10.1016/j.actatropica.2016.03.035